Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.
Identifieur interne : 003053 ( Main/Exploration ); précédent : 003052; suivant : 003054Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.
Auteurs : Fumihiko Yasui [Japon] ; Chieko Kai ; Masahiro Kitabatake ; Shingo Inoue ; Misako Yoneda ; Shoji Yokochi ; Ryoichi Kase ; Satoshi Sekiguchi ; Kouichi Morita ; Tsunekazu Hishima ; Hidenori Suzuki ; Katsuo Karamatsu ; Yasuhiro Yasutomi ; Hisatoshi Shida ; Minoru Kidokoro ; Kyosuke Mizuno ; Kouji Matsushima ; Michinori KoharaSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 1550-6606 ] ; 2008.
Descripteurs français
- KwdFr :
- ARN messager (biosynthèse), Animaux, Anticorps antiviraux (biosynthèse), Cellules Vero, Cytokines (biosynthèse), Femelle, Humains, Indice de gravité médicale, Lapins, Lignée cellulaire, Pneumopathie virale (anatomopathologie), Pneumopathie virale (immunologie), Pneumopathie virale (virologie), Poumon (anatomopathologie), Poumon (immunologie), Poumon (virologie), Protéines nucléocapside (administration et posologie), Protéines nucléocapside (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (virologie), Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Protéines nucléocapside, Vaccins antiviraux.
- anatomopathologie : Pneumopathie virale, Poumon, Syndrome respiratoire aigu sévère.
- biosynthèse : ARN messager, Anticorps antiviraux, Cytokines.
- immunologie : Pneumopathie virale, Poumon, Protéines nucléocapside, Vaccins antiviraux, Virus du SRAS.
- virologie : Pneumopathie virale, Poumon, Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Femelle, Humains, Indice de gravité médicale, Lapins, Lignée cellulaire, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (biosynthesis), Cell Line, Chlorocebus aethiops, Cytokines (biosynthesis), Female, Humans, Lung (immunology), Lung (pathology), Lung (virology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (administration & dosage), Nucleocapsid Proteins (immunology), Pneumonia, Viral (immunology), Pneumonia, Viral (pathology), Pneumonia, Viral (virology), RNA, Messenger (biosynthesis), Rabbits, SARS Virus (immunology), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Severity of Illness Index, Vero Cells, Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Nucleocapsid Proteins, Viral Vaccines.
- chemical , biosynthesis : Antibodies, Viral, Cytokines, RNA, Messenger.
- immunology : Lung, Nucleocapsid Proteins, Pneumonia, Viral, SARS Virus, Viral Vaccines.
- pathology : Lung, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Lung, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Chlorocebus aethiops, Female, Humans, Mice, Mice, Inbred BALB C, Rabbits, Severity of Illness Index, Vero Cells.
Abstract
The details of the mechanism by which severe acute respiratory syndrome-associated coronavirus (SARS-CoV) causes severe pneumonia are unclear. We investigated the immune responses and pathologies of SARS-CoV-infected BALB/c mice that were immunized intradermally with recombinant vaccinia virus (VV) that expressed either the SARS-CoV spike (S) protein (LC16m8rVV-S) or simultaneously all the structural proteins, including the nucleocapsid (N), membrane (M), envelope (E), and S proteins (LC16m8rVV-NMES) 7-8 wk before intranasal SARS-CoV infection. The LC16m8rVV-NMES-immunized group exhibited as severe pneumonia as the control groups, although LC16m8rVV-NMES significantly decreased the pulmonary SARS-CoV titer to the same extent as LC16m8rVV-S. To identify the cause of the exacerbated pneumonia, BALB/c mice were immunized with recombinant VV that expressed the individual structural proteins of SARS-CoV (LC16mOrVV-N, -M, -E, -S) with or without LC16mOrVV-S (i.e., LC16mOrVV-N, LC16mOrVV-M, LC16mOrVV-E, or LC16mOrVV-S alone or LC16mOrVV-N + LC16mOrVV-S, LC16mOrVV-M + LC16mOrVV-S, or LC16mOrVV-E + LC16mOrVV-S), and infected with SARS-CoV more than 4 wk later. Both LC16mOrVV-N-immunized mice and LC16mOrVV-N + LC16mOrVV-S-immunized mice exhibited severe pneumonia. Furthermore, LC16mOrVV-N-immunized mice upon infection exhibited significant up-regulation of both Th1 (IFN-gamma, IL-2) and Th2 (IL-4, IL-5) cytokines and down-regulation of anti-inflammatory cytokines (IL-10, TGF-beta), resulting in robust infiltration of neutrophils, eosinophils, and lymphocytes into the lung, as well as thickening of the alveolar epithelium. These results suggest that an excessive host immune response against the nucleocapsid protein of SARS-CoV is involved in severe pneumonia caused by SARS-CoV infection. These findings increase our understanding of the pathogenesis of SARS.
DOI: 10.4049/jimmunol.181.9.6337
PubMed: 18941225
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A46
- to stream PubMed, to step Curation: 001A46
- to stream PubMed, to step Checkpoint: 001A58
- to stream Ncbi, to step Merge: 001D71
- to stream Ncbi, to step Curation: 001D71
- to stream Ncbi, to step Checkpoint: 001D71
- to stream Main, to step Merge: 003126
- to stream Main, to step Curation: 003053
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.</title><author><name sortKey="Yasui, Fumihiko" sort="Yasui, Fumihiko" uniqKey="Yasui F" first="Fumihiko" last="Yasui">Fumihiko Yasui</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science</wicri:regionArea><wicri:noRegion>The Tokyo Metropolitan Institute of Medical Science</wicri:noRegion></affiliation></author><author><name sortKey="Kai, Chieko" sort="Kai, Chieko" uniqKey="Kai C" first="Chieko" last="Kai">Chieko Kai</name></author><author><name sortKey="Kitabatake, Masahiro" sort="Kitabatake, Masahiro" uniqKey="Kitabatake M" first="Masahiro" last="Kitabatake">Masahiro Kitabatake</name></author><author><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name></author><author><name sortKey="Yoneda, Misako" sort="Yoneda, Misako" uniqKey="Yoneda M" first="Misako" last="Yoneda">Misako Yoneda</name></author><author><name sortKey="Yokochi, Shoji" sort="Yokochi, Shoji" uniqKey="Yokochi S" first="Shoji" last="Yokochi">Shoji Yokochi</name></author><author><name sortKey="Kase, Ryoichi" sort="Kase, Ryoichi" uniqKey="Kase R" first="Ryoichi" last="Kase">Ryoichi Kase</name></author><author><name sortKey="Sekiguchi, Satoshi" sort="Sekiguchi, Satoshi" uniqKey="Sekiguchi S" first="Satoshi" last="Sekiguchi">Satoshi Sekiguchi</name></author><author><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name></author><author><name sortKey="Hishima, Tsunekazu" sort="Hishima, Tsunekazu" uniqKey="Hishima T" first="Tsunekazu" last="Hishima">Tsunekazu Hishima</name></author><author><name sortKey="Suzuki, Hidenori" sort="Suzuki, Hidenori" uniqKey="Suzuki H" first="Hidenori" last="Suzuki">Hidenori Suzuki</name></author><author><name sortKey="Karamatsu, Katsuo" sort="Karamatsu, Katsuo" uniqKey="Karamatsu K" first="Katsuo" last="Karamatsu">Katsuo Karamatsu</name></author><author><name sortKey="Yasutomi, Yasuhiro" sort="Yasutomi, Yasuhiro" uniqKey="Yasutomi Y" first="Yasuhiro" last="Yasutomi">Yasuhiro Yasutomi</name></author><author><name sortKey="Shida, Hisatoshi" sort="Shida, Hisatoshi" uniqKey="Shida H" first="Hisatoshi" last="Shida">Hisatoshi Shida</name></author><author><name sortKey="Kidokoro, Minoru" sort="Kidokoro, Minoru" uniqKey="Kidokoro M" first="Minoru" last="Kidokoro">Minoru Kidokoro</name></author><author><name sortKey="Mizuno, Kyosuke" sort="Mizuno, Kyosuke" uniqKey="Mizuno K" first="Kyosuke" last="Mizuno">Kyosuke Mizuno</name></author><author><name sortKey="Matsushima, Kouji" sort="Matsushima, Kouji" uniqKey="Matsushima K" first="Kouji" last="Matsushima">Kouji Matsushima</name></author><author><name sortKey="Kohara, Michinori" sort="Kohara, Michinori" uniqKey="Kohara M" first="Michinori" last="Kohara">Michinori Kohara</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18941225</idno><idno type="pmid">18941225</idno><idno type="doi">10.4049/jimmunol.181.9.6337</idno><idno type="wicri:Area/PubMed/Corpus">001A46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A46</idno><idno type="wicri:Area/PubMed/Curation">001A46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A46</idno><idno type="wicri:Area/PubMed/Checkpoint">001A58</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A58</idno><idno type="wicri:Area/Ncbi/Merge">001D71</idno><idno type="wicri:Area/Ncbi/Curation">001D71</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D71</idno><idno type="wicri:Area/Main/Merge">003126</idno><idno type="wicri:Area/Main/Curation">003053</idno><idno type="wicri:Area/Main/Exploration">003053</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.</title><author><name sortKey="Yasui, Fumihiko" sort="Yasui, Fumihiko" uniqKey="Yasui F" first="Fumihiko" last="Yasui">Fumihiko Yasui</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology and Cell Biology, The Tokyo Metropolitan Institute of Medical Science</wicri:regionArea><wicri:noRegion>The Tokyo Metropolitan Institute of Medical Science</wicri:noRegion></affiliation></author><author><name sortKey="Kai, Chieko" sort="Kai, Chieko" uniqKey="Kai C" first="Chieko" last="Kai">Chieko Kai</name></author><author><name sortKey="Kitabatake, Masahiro" sort="Kitabatake, Masahiro" uniqKey="Kitabatake M" first="Masahiro" last="Kitabatake">Masahiro Kitabatake</name></author><author><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name></author><author><name sortKey="Yoneda, Misako" sort="Yoneda, Misako" uniqKey="Yoneda M" first="Misako" last="Yoneda">Misako Yoneda</name></author><author><name sortKey="Yokochi, Shoji" sort="Yokochi, Shoji" uniqKey="Yokochi S" first="Shoji" last="Yokochi">Shoji Yokochi</name></author><author><name sortKey="Kase, Ryoichi" sort="Kase, Ryoichi" uniqKey="Kase R" first="Ryoichi" last="Kase">Ryoichi Kase</name></author><author><name sortKey="Sekiguchi, Satoshi" sort="Sekiguchi, Satoshi" uniqKey="Sekiguchi S" first="Satoshi" last="Sekiguchi">Satoshi Sekiguchi</name></author><author><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name></author><author><name sortKey="Hishima, Tsunekazu" sort="Hishima, Tsunekazu" uniqKey="Hishima T" first="Tsunekazu" last="Hishima">Tsunekazu Hishima</name></author><author><name sortKey="Suzuki, Hidenori" sort="Suzuki, Hidenori" uniqKey="Suzuki H" first="Hidenori" last="Suzuki">Hidenori Suzuki</name></author><author><name sortKey="Karamatsu, Katsuo" sort="Karamatsu, Katsuo" uniqKey="Karamatsu K" first="Katsuo" last="Karamatsu">Katsuo Karamatsu</name></author><author><name sortKey="Yasutomi, Yasuhiro" sort="Yasutomi, Yasuhiro" uniqKey="Yasutomi Y" first="Yasuhiro" last="Yasutomi">Yasuhiro Yasutomi</name></author><author><name sortKey="Shida, Hisatoshi" sort="Shida, Hisatoshi" uniqKey="Shida H" first="Hisatoshi" last="Shida">Hisatoshi Shida</name></author><author><name sortKey="Kidokoro, Minoru" sort="Kidokoro, Minoru" uniqKey="Kidokoro M" first="Minoru" last="Kidokoro">Minoru Kidokoro</name></author><author><name sortKey="Mizuno, Kyosuke" sort="Mizuno, Kyosuke" uniqKey="Mizuno K" first="Kyosuke" last="Mizuno">Kyosuke Mizuno</name></author><author><name sortKey="Matsushima, Kouji" sort="Matsushima, Kouji" uniqKey="Matsushima K" first="Kouji" last="Matsushima">Kouji Matsushima</name></author><author><name sortKey="Kohara, Michinori" sort="Kohara, Michinori" uniqKey="Kohara M" first="Michinori" last="Kohara">Michinori Kohara</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="eISSN">1550-6606</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cytokines (biosynthesis)</term><term>Female</term><term>Humans</term><term>Lung (immunology)</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (administration & dosage)</term><term>Nucleocapsid Proteins (immunology)</term><term>Pneumonia, Viral (immunology)</term><term>Pneumonia, Viral (pathology)</term><term>Pneumonia, Viral (virology)</term><term>RNA, Messenger (biosynthesis)</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severity of Illness Index</term><term>Vero Cells</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (biosynthèse)</term><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Cellules Vero</term><term>Cytokines (biosynthèse)</term><term>Femelle</term><term>Humains</term><term>Indice de gravité médicale</term><term>Lapins</term><term>Lignée cellulaire</term><term>Pneumopathie virale (anatomopathologie)</term><term>Pneumopathie virale (immunologie)</term><term>Pneumopathie virale (virologie)</term><term>Poumon (anatomopathologie)</term><term>Poumon (immunologie)</term><term>Poumon (virologie)</term><term>Protéines nucléocapside (administration et posologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term><term>Cytokines</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Pneumopathie virale</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>Anticorps antiviraux</term><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Pneumopathie virale</term><term>Poumon</term><term>Protéines nucléocapside</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lung</term><term>Nucleocapsid Proteins</term><term>Pneumonia, Viral</term><term>SARS Virus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung</term><term>Pneumonia, Viral</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Pneumopathie virale</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Pneumonia, Viral</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Rabbits</term><term>Severity of Illness Index</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Femelle</term><term>Humains</term><term>Indice de gravité médicale</term><term>Lapins</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The details of the mechanism by which severe acute respiratory syndrome-associated coronavirus (SARS-CoV) causes severe pneumonia are unclear. We investigated the immune responses and pathologies of SARS-CoV-infected BALB/c mice that were immunized intradermally with recombinant vaccinia virus (VV) that expressed either the SARS-CoV spike (S) protein (LC16m8rVV-S) or simultaneously all the structural proteins, including the nucleocapsid (N), membrane (M), envelope (E), and S proteins (LC16m8rVV-NMES) 7-8 wk before intranasal SARS-CoV infection. The LC16m8rVV-NMES-immunized group exhibited as severe pneumonia as the control groups, although LC16m8rVV-NMES significantly decreased the pulmonary SARS-CoV titer to the same extent as LC16m8rVV-S. To identify the cause of the exacerbated pneumonia, BALB/c mice were immunized with recombinant VV that expressed the individual structural proteins of SARS-CoV (LC16mOrVV-N, -M, -E, -S) with or without LC16mOrVV-S (i.e., LC16mOrVV-N, LC16mOrVV-M, LC16mOrVV-E, or LC16mOrVV-S alone or LC16mOrVV-N + LC16mOrVV-S, LC16mOrVV-M + LC16mOrVV-S, or LC16mOrVV-E + LC16mOrVV-S), and infected with SARS-CoV more than 4 wk later. Both LC16mOrVV-N-immunized mice and LC16mOrVV-N + LC16mOrVV-S-immunized mice exhibited severe pneumonia. Furthermore, LC16mOrVV-N-immunized mice upon infection exhibited significant up-regulation of both Th1 (IFN-gamma, IL-2) and Th2 (IL-4, IL-5) cytokines and down-regulation of anti-inflammatory cytokines (IL-10, TGF-beta), resulting in robust infiltration of neutrophils, eosinophils, and lymphocytes into the lung, as well as thickening of the alveolar epithelium. These results suggest that an excessive host immune response against the nucleocapsid protein of SARS-CoV is involved in severe pneumonia caused by SARS-CoV infection. These findings increase our understanding of the pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Hishima, Tsunekazu" sort="Hishima, Tsunekazu" uniqKey="Hishima T" first="Tsunekazu" last="Hishima">Tsunekazu Hishima</name><name sortKey="Inoue, Shingo" sort="Inoue, Shingo" uniqKey="Inoue S" first="Shingo" last="Inoue">Shingo Inoue</name><name sortKey="Kai, Chieko" sort="Kai, Chieko" uniqKey="Kai C" first="Chieko" last="Kai">Chieko Kai</name><name sortKey="Karamatsu, Katsuo" sort="Karamatsu, Katsuo" uniqKey="Karamatsu K" first="Katsuo" last="Karamatsu">Katsuo Karamatsu</name><name sortKey="Kase, Ryoichi" sort="Kase, Ryoichi" uniqKey="Kase R" first="Ryoichi" last="Kase">Ryoichi Kase</name><name sortKey="Kidokoro, Minoru" sort="Kidokoro, Minoru" uniqKey="Kidokoro M" first="Minoru" last="Kidokoro">Minoru Kidokoro</name><name sortKey="Kitabatake, Masahiro" sort="Kitabatake, Masahiro" uniqKey="Kitabatake M" first="Masahiro" last="Kitabatake">Masahiro Kitabatake</name><name sortKey="Kohara, Michinori" sort="Kohara, Michinori" uniqKey="Kohara M" first="Michinori" last="Kohara">Michinori Kohara</name><name sortKey="Matsushima, Kouji" sort="Matsushima, Kouji" uniqKey="Matsushima K" first="Kouji" last="Matsushima">Kouji Matsushima</name><name sortKey="Mizuno, Kyosuke" sort="Mizuno, Kyosuke" uniqKey="Mizuno K" first="Kyosuke" last="Mizuno">Kyosuke Mizuno</name><name sortKey="Morita, Kouichi" sort="Morita, Kouichi" uniqKey="Morita K" first="Kouichi" last="Morita">Kouichi Morita</name><name sortKey="Sekiguchi, Satoshi" sort="Sekiguchi, Satoshi" uniqKey="Sekiguchi S" first="Satoshi" last="Sekiguchi">Satoshi Sekiguchi</name><name sortKey="Shida, Hisatoshi" sort="Shida, Hisatoshi" uniqKey="Shida H" first="Hisatoshi" last="Shida">Hisatoshi Shida</name><name sortKey="Suzuki, Hidenori" sort="Suzuki, Hidenori" uniqKey="Suzuki H" first="Hidenori" last="Suzuki">Hidenori Suzuki</name><name sortKey="Yasutomi, Yasuhiro" sort="Yasutomi, Yasuhiro" uniqKey="Yasutomi Y" first="Yasuhiro" last="Yasutomi">Yasuhiro Yasutomi</name><name sortKey="Yokochi, Shoji" sort="Yokochi, Shoji" uniqKey="Yokochi S" first="Shoji" last="Yokochi">Shoji Yokochi</name><name sortKey="Yoneda, Misako" sort="Yoneda, Misako" uniqKey="Yoneda M" first="Misako" last="Yoneda">Misako Yoneda</name></noCountry><country name="Japon"><noRegion><name sortKey="Yasui, Fumihiko" sort="Yasui, Fumihiko" uniqKey="Yasui F" first="Fumihiko" last="Yasui">Fumihiko Yasui</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003053 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003053 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18941225
|texte= Prior immunization with severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18941225" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |